3ZCO
Crystal structure of S. cerevisiae Sir3 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 75.320, 75.320, 55.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.130 - 2.700 |
| R-factor | 0.2181 |
| Rwork | 0.211 |
| R-free | 0.26260 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 5076 | |
| <I/σ(I)> | 25.7 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000, 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM CITRATE PH 5.6 |
