3ZCF
Structure of recombinant human cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.545, 53.915, 58.723 |
| Unit cell angles | 76.54, 88.57, 72.10 |
Refinement procedure
| Resolution | 49.900 - 1.650 |
| R-factor | 0.17437 |
| Rwork | 0.172 |
| R-free | 0.21182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nwv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.270 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 46913 | |
| <I/σ(I)> | 8.5 | 2.3 |
| Completeness [%] | 94.1 | 83.1 |
| Redundancy | 2.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 26 TO 31 % W/V POLYETHYLENE GLYCOL 1000 AND 40 MM KH2PO4, PH 7.0 |
