3ZCD
Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.180, 98.630, 131.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.840 - 1.550 |
| R-factor | 0.15362 |
| Rwork | 0.151 |
| R-free | 0.20378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lji |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.540 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.100 | 0.360 |
| Number of reflections | 78917 | |
| <I/σ(I)> | 7.2 | 3.4 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG3350, 0.2 M NAF, 0.1 M BISTRISPROPANE PH 6.5 |
