3ZCB
VbhT Fic protein from Bartonella schoenbuchensis in complex with VbhA antitoxin mutant E24G and ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-15 |
| Detector | DECTRIS PILATUS-2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.460, 40.250, 73.900 |
| Unit cell angles | 90.00, 121.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.940 |
| R-factor | 0.1978 |
| Rwork | 0.195 |
| R-free | 0.24931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3shg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.332 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.440 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 19937 | |
| <I/σ(I)> | 14.39 | 3.34 |
| Completeness [%] | 98.8 | 97.7 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 16% W/V PEG4000, 0.1M MES PH6.5 |
