3ZBZ

Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation V321A, crystallized with 2'-AMPS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-01
Detector	MARRESEARCH SX-165
Spacegroup name	P 21 21 21
Unit cell lengths	41.250, 46.990, 107.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.474 - 2.100
R-factor	0.2104
Rwork	0.207
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmi
RMSD bond length	0.002
RMSD bond angle	0.594
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.130	0.870
Number of reflections	12529
<I/σ(I)>	9.9	1.9
Completeness [%]	97.8	93
Redundancy	4.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4	277	250 UM PROTEIN WITH 10 MM 23-RP-CYCLIC AMPS WAS MIXED IN 0.5 PLUS 0.5 UL DROPS WITH 30% PEG4000 AND 50 MM ACETATE PH 3 IN 4 DEG C