3ZBR
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation H230S, crystallized with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-02 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.100, 47.100, 111.170 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 29.123 - 2.304 |
| R-factor | 0.2264 |
| Rwork | 0.220 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 1.140 |
| Number of reflections | 19310 | |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 98.6 | 91.2 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 277 | 250 UM PROTEIN WITH 10 MM 23-CNADP MIXED IN 0.5 PLUS 0.5 UL DROPS WITH 50 MM NA-FORMATE (1:2 PH3:PH5) AND 23% PEG 4000 IN 4 DEGC |
