3ZBP
Monomeric subunit of TubZ from Bacteriophage PhiKZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.453, 76.275, 54.065 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 44.108 - 2.000 |
| R-factor | 0.1742 |
| Rwork | 0.172 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r4v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.110 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.540 |
| Number of reflections | 26417 | |
| <I/σ(I)> | 8.9 | 2.6 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 8% ETHYLENE GLYCOL, 20% PEG5KMME, 0.15 M TRIS-CL PH 8.0 |
