3ZBJ
Fitting results in the I-layer of the subnanometer structure of the bacterial pKM101 type IV secretion system core complex digested with elastase
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TECNAI 12 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.1 |
| Detector | GENERIC GATAN |
| Number of Images | 262 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.5 |
| Software | Flex-EM,Sculptor,Situs,UCSF Chimera,IMAGIC |
| Symmetry | C14 |
