3X3X
Copper amine oxidase from Arthrobacter globiformis anaerobically reduced by phenylethylamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-28 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 191.658, 62.890, 158.006 |
Unit cell angles | 90.00, 117.48, 90.00 |
Refinement procedure
Resolution | 20.414 - 1.570 |
R-factor | 0.2163 |
Rwork | 0.215 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.053 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.519 | 1.650 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.093 | 0.392 |
Number of reflections | 228324 | |
<I/σ(I)> | 16.7 | 1.9 |
Completeness [%] | 98.1 | 96 |
Redundancy | 6.6 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |