3X3Q
Structure of recombinant thaumatin in the presence of 1.0M PST, pH7 at 293K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-01-19 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.459, 58.459, 151.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.701 - 1.500 |
| R-factor | 0.1083 |
| Rwork | 0.107 |
| R-free | 0.13660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.460 |
| Number of reflections | 45837 | |
| <I/σ(I)> | 71.36 | 7.56 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.05M ADA, 1.0M K-Na TARTRATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
