3X3G
Fab fragment from anti TRAIL-R2 Human Agonist Antibody KMTR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 153.001, 165.383, 65.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.250 - 2.510 |
R-factor | 0.18777 |
Rwork | 0.186 |
R-free | 0.22411 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x3f |
RMSD bond length | 0.012 |
RMSD bond angle | 1.701 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.300 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 27370 | |
<I/σ(I)> | 27.6 | 3.8 |
Completeness [%] | 95.4 | 81.1 |
Redundancy | 6.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 20% PEG3350, 0.2M sodium sulfate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |