3X38
Crystal structure of the C-terminal domain of Sld7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.094, 60.538, 93.130 |
Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
Resolution | 34.007 - 1.801 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.029 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 35136 | |
<I/σ(I)> | 4.2 | |
Completeness [%] | 98.5 | 99.8 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M Acetate-NaOH, 0.1M LiSO4, 12.5% PEG 8K, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |