3X37
Crystal structure of the N-terminal domain of Sld7 in complex with Sld3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97865 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.706, 37.860, 98.404 |
| Unit cell angles | 90.00, 126.79, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.350 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.24450 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.140 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 15781 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.1M CHES-NaOH, 0.8M Na-Citrate, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
