3X34
Crystal structure of the reduced form of the solubilized domain of porcine cytochrome b5 in form 1 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.80 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.993, 38.874, 60.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.760 |
R-factor | 0.107 |
Rwork | 0.106 |
R-free | 0.13100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cyo |
RMSD bond length | 0.018 |
RMSD bond angle | 0.043 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.770 |
High resolution limit [Å] | 0.760 | 0.760 |
Rmerge | 0.071 | 0.386 |
Number of reflections | 99196 | |
<I/σ(I)> | 67.4 | 4.1 |
Completeness [%] | 99.8 | 97.3 |
Redundancy | 12.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 24% PEG 1500, 4% 2-propanol, 0.1M calcium chloride, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |