3X33
Crystal structure of the oxidized form of the solubilized domain of porcine cytochrome b5 in form 2 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.559, 51.175, 55.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.930 |
R-factor | 0.13 |
Rwork | 0.129 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cyo |
RMSD bond length | 0.015 |
RMSD bond angle | 0.043 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.950 |
High resolution limit [Å] | 0.930 | 0.930 |
Rmerge | 0.068 | 0.435 |
Number of reflections | 63824 | |
<I/σ(I)> | 50.9 | 3.5 |
Completeness [%] | 98.8 | 96.7 |
Redundancy | 6.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15% PEG 400, 3% PEG 3350, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |