3X32
Crystal structure of the oxidized form of the solubilized domain of porcine cytochrome b5 in form 1 crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.91 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.339, 39.279, 60.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.830 |
R-factor | 0.107 |
Rwork | 0.106 |
R-free | 0.12700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cyo |
RMSD bond length | 0.037 |
RMSD bond angle | 0.040 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.840 |
High resolution limit [Å] | 0.830 | 0.830 |
Rmerge | 0.089 | 0.374 |
Number of reflections | 72789 | |
<I/σ(I)> | 59.8 | 7.6 |
Completeness [%] | 98.2 | 82.3 |
Redundancy | 15.5 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 24% PEG 1500, 4% 2-propanol, 0.1M calcium chloride, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |