3X32
Crystal structure of the oxidized form of the solubilized domain of porcine cytochrome b5 in form 1 crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.339, 39.279, 60.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.830 |
| R-factor | 0.107 |
| Rwork | 0.106 |
| R-free | 0.12700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cyo |
| RMSD bond length | 0.037 |
| RMSD bond angle | 0.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.840 |
| High resolution limit [Å] | 0.830 | 0.830 |
| Rmerge | 0.089 | 0.374 |
| Number of reflections | 72789 | |
| <I/σ(I)> | 59.8 | 7.6 |
| Completeness [%] | 98.2 | 82.3 |
| Redundancy | 15.5 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 24% PEG 1500, 4% 2-propanol, 0.1M calcium chloride, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
