3X1O
Crystal structure of the ROQ domain of human Roquin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95350 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.003, 78.322, 184.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.497 - 2.201 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.25210 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.928 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.550 | |
| Number of reflections | 20113 | |
| <I/σ(I)> | 17.4 | 2.8 |
| Completeness [%] | 97.4 | 90.3 |
| Redundancy | 8.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M lithium chloride, 20% Glycol3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
