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3X17

Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL44XU
Synchrotron siteSPring-8
BeamlineBL44XU
Temperature [K]100
Detector technologyCCD
Collection date2013-12-13
DetectorRAYONIX MX225HE
Wavelength(s)0.9
Spacegroup nameP 21 21 21
Unit cell lengths84.631, 89.913, 151.157
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution43.960 - 2.150
R-factor0.18978
Rwork0.187
R-free0.24558
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1clc
RMSD bond length0.011
RMSD bond angle1.190
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]2.150
Rmerge0.133
Number of reflections63355
<I/σ(I)>28
Completeness [%]99.9
Redundancy14.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.52930.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, 30% w/v Polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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