3X17
Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.631, 89.913, 151.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.960 - 2.150 |
R-factor | 0.18978 |
Rwork | 0.187 |
R-free | 0.24558 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clc |
RMSD bond length | 0.011 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.133 |
Number of reflections | 63355 |
<I/σ(I)> | 28 |
Completeness [%] | 99.9 |
Redundancy | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, 30% w/v Polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |