3X17
Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.631, 89.913, 151.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.960 - 2.150 |
| R-factor | 0.18978 |
| Rwork | 0.187 |
| R-free | 0.24558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.133 |
| Number of reflections | 63355 |
| <I/σ(I)> | 28 |
| Completeness [%] | 99.9 |
| Redundancy | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, 30% w/v Polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
