3X15
Dimeric Aquifex aeolicus cytochrome c555
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.591, 54.464, 65.403 |
| Unit cell angles | 90.00, 114.47, 90.00 |
Refinement procedure
| Resolution | 33.121 - 1.600 |
| R-factor | 0.15378 |
| Rwork | 0.150 |
| R-free | 0.21377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.599 |
| Number of reflections | 47953 | |
| <I/σ(I)> | 24.794 | 2.019 |
| Completeness [%] | 98.7 | 97.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M ammonium sulfate, 0.1M Sodium cacodylate trihydrate, 30% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
