3X0W
Crystal structure of PLEKHM1 LIR-fused human LC3B_2-119
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.009, 69.009, 119.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.710 |
| R-factor | 0.22752 |
| Rwork | 0.224 |
| R-free | 0.29384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.860 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Number of reflections | 7759 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 92.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Acetate, pH 5.0, 1.4M Ammonium Sulfate, 0.1M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
