3WZT
Crystal structure of Trx3 domain of UGGT (detergent-unbound form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-08-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97888 |
Spacegroup name | I 2 3 |
Unit cell lengths | 196.400, 196.400, 196.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.400 |
R-factor | 0.237 |
Rwork | 0.235 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wzs |
RMSD bond length | 0.010 |
RMSD bond angle | 1.279 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.127 | 0.677 |
Number of reflections | 17411 | |
<I/σ(I)> | 34.1 | 6.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 44.7 | 45.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 60% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |