3WZN
Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin at 1.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.677, 54.344, 60.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.300 |
| R-factor | 0.16239 |
| Rwork | 0.160 |
| R-free | 0.20658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wyq |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 54829 | |
| <I/σ(I)> | 26.8 | 3.95 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 100 mM sodium acetate trihydrate, 2.0 M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
