3WZ3
Structure of a periplasmic fragment of TraM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.757, 67.757, 73.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.956 - 1.500 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.20680 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.900 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.115 | 0.351 |
| Number of reflections | 27756 | |
| <I/σ(I)> | 17.4 | 8.9 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 21.1 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.05M Glycine pH9.0, 0.8M Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
