3WYH

Structure of disulfide bond deletion mutant of ostrich egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-16
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	38.188, 64.934, 131.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 1.770
Rwork	0.186
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	153l
RMSD bond length	0.018
RMSD bond angle	1.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.770	1.770
Number of reflections	29391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	50% PEG 200, 0.05M Li2SO4, 0.1M tris-HCl , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K