3WYF
Crystal structure of Xpo1p-Yrb2p-Gsp1p-GTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.490, 155.950, 149.820 |
Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
Resolution | 33.690 - 2.220 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m1i |
RMSD bond length | 0.013 |
RMSD bond angle | 1.695 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.690 | 2.260 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 187333 | |
Completeness [%] | 99.0 | 90.6 |
Redundancy | 3.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 0.1M CITRATE, 17% PEG6000, PH 5.7, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |