3WY1
Crystal structure of alpha-glucosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.540, 119.590, 177.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.493 - 2.150 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m53 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1391)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.300 | 99.300 | 2.280 |
| High resolution limit [Å] | 2.150 | 6.390 | 2.150 |
| Rmerge | 0.083 | 0.028 | 0.489 |
| Number of reflections | 71192 | ||
| <I/σ(I)> | 20.8 | 52.82 | 5.75 |
| Completeness [%] | 99.7 | 99.1 | 98.7 |
| Redundancy | 7.3 | 6.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% Polyacrylic acid 5100 sodium salt, 0.1M HEPES, 0.02M magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
