3WXR
Yeast 20S proteasome with a mutation of alpha7 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.248, 298.622, 145.401 |
| Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
| Resolution | 48.200 - 3.150 |
| R-factor | 0.17717 |
| Rwork | 0.174 |
| R-free | 0.24208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.135 | 0.422 |
| Number of reflections | 183783 | |
| <I/σ(I)> | 13.66 | 3.461 |
| Completeness [%] | 87.0 | 82.2 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% MPD, 0.1M MES, 50mM Magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
