3WXE
Crystal structure of CYLD USP domain (C596S) in complex with Met1-linked diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-20 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.676, 49.788, 71.003 |
Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.18518 |
Rwork | 0.182 |
R-free | 0.24722 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2VHF and 1UBQ |
RMSD bond length | 0.016 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 15549 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 96.9 | 92.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 50mM Tris-HCl buffer, 9% PEG4000, 100mM magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |