3WXB
Crystal structure of NADPH bound carbonyl reductase from chicken fatty liver
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 74.568, 74.568, 190.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.590 - 1.980 |
Rwork | 0.215 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yo6 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.980 |
Number of reflections | 43916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 23% PEG 5000 MME, 0.1M Bis-Tris buffer (pH 6.5) , VAPOR DIFFUSION, SITTING DROP, temperature 293K |