3WXA
X-ray crystal structural analysis of the complex between ALG-2 and Sec31A peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.28209 |
| Spacegroup name | P 64 |
| Unit cell lengths | 81.973, 81.973, 103.232 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.094 - 2.360 |
| R-factor | 0.2304 |
| Rwork | 0.225 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2znd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.612 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.000 | 2.421 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 16160 | |
| <I/σ(I)> | 33 | 33 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 10.3 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M Cacodylate, 20% MPD, 0.05M Zinc Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
