3WXA
X-ray crystal structural analysis of the complex between ALG-2 and Sec31A peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.28209 |
Spacegroup name | P 64 |
Unit cell lengths | 81.973, 81.973, 103.232 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.094 - 2.360 |
R-factor | 0.2304 |
Rwork | 0.225 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2znd |
RMSD bond length | 0.003 |
RMSD bond angle | 0.612 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.000 | 2.421 |
High resolution limit [Å] | 2.360 | 2.360 |
Number of reflections | 16160 | |
<I/σ(I)> | 33 | 33 |
Completeness [%] | 99.4 | 99.4 |
Redundancy | 10.3 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M Cacodylate, 20% MPD, 0.05M Zinc Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |