3WX6
Crystal structure of Type Six Secretion System protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 6 |
| Unit cell lengths | 82.738, 82.738, 64.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.937 - 2.700 |
| R-factor | 0.2536 |
| Rwork | 0.249 |
| R-free | 0.28460 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.219 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 6995 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 29.5 | 29.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M NaHEPES, 1M tri-sodiumcitrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
