3WX5
Crystal structure of metagenome-derived glycoside hydrolase family 12 endoglucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.779, 59.576, 74.950 |
| Unit cell angles | 90.00, 122.89, 90.00 |
Refinement procedure
| Resolution | 33.360 - 1.850 |
| R-factor | 0.16171 |
| Rwork | 0.160 |
| R-free | 0.20008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0b |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.120 |
| Number of reflections | 41281 |
| <I/σ(I)> | 21.8 |
| Completeness [%] | 99.4 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 0.2M sodium chloride, 23% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
