3WWT

Crystal Structure of the Y3:STAT1ND complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	72.270, 72.270, 197.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.020 - 2.000
R-factor	0.2066
Rwork	0.205
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yvl
RMSD bond length	0.007
RMSD bond angle	0.784
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660+SVN)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.074	0.052	0.432
Number of reflections	21429	2272	2100
<I/σ(I)>	9.1
Completeness [%]	98.4	93.1	99.6
Redundancy	11.8	12.4	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	30% PEG400, 0.2M CaCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K