3WWN
Crystal structure of LysZ from Thermus thermophilus complex with LysW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-11-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.809, 69.809, 148.036 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.770 - 1.850 |
R-factor | 0.17876 |
Rwork | 0.177 |
R-free | 0.21282 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3WWL and 3U6U |
RMSD bond length | 0.008 |
RMSD bond angle | 1.314 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 32177 | |
<I/σ(I)> | 46.9 | 3.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 14 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% (v/w) PEG 8000, 0.1M MES pH 6.0, 0.2M Calcium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |