3WW6
Crystal Structure of hen egg white lysozyme mutant N46D/D52S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.006, 77.006, 37.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.748 - 1.530 |
| R-factor | 0.1878 |
| Rwork | 0.187 |
| R-free | 0.20520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lzt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
| High resolution limit [Å] | 1.530 | 3.300 | 1.530 |
| Rmerge | 0.073 | 0.055 | 0.211 |
| Number of reflections | 17242 | ||
| <I/σ(I)> | 76.659 | ||
| Completeness [%] | 98.8 | 91.3 | 99.9 |
| Redundancy | 13.1 | 11.8 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.6M sodium chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
