3WW5
Crystal Structure of hen egg white lysozyme mutant N46E/D52S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 76.056, 76.056, 36.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.100 - 1.530 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lzt |
RMSD bond length | 0.006 |
RMSD bond angle | 1.056 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
High resolution limit [Å] | 1.530 | 3.300 | 1.530 |
Rmerge | 0.089 | 0.063 | 0.288 |
Number of reflections | 16843 | ||
<I/σ(I)> | 77.485 | ||
Completeness [%] | 99.7 | 97.1 | 100 |
Redundancy | 13.8 | 12.2 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.6M sodium chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |