3WW0

Crystal structure of F97A mutant, a new nuclear transport receptor of Hsp70

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	AREA DETECTOR
Collection date	2013-11-08
Wavelength(s)	0.9192
Spacegroup name	P 32
格子定数 [Å]	85.483, 85.483, 68.943
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	42.741 - 2.500
R因子	0.1969
Rwork	0.196
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.735
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (AutoSol)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.500
独立反射数	12674

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.4	293.15	0.1M Tris-HCl (pH 7.4), 1M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K