3WVS
Crystal Structure of Cytochrome P450revI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.779, 73.472, 58.305 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 27.010 - 1.400 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.18840 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.161 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (refmac_5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.400 |
| Number of reflections | 81049 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG3350, Tartrate, pH 7.5, vapor diffusion, temperature 298K |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG3350, Tartrate, pH 7.5, vapor diffusion, temperature 298K |
