3WVO
Crystal structure of Thermobifida fusca Cse1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.304, 132.529, 102.962 |
| Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
| Resolution | 48.370 - 3.310 |
| R-factor | 0.15895 |
| Rwork | 0.156 |
| R-free | 0.20974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h3t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.797 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 35245 | |
| Completeness [%] | 89.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG8000, Cacodylate, potassium chloride, magnesium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
