3WVL
Crystal structure of the football-shaped GroEL-GroES complex (GroEL: GroES2:ATP14) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 160.280, 217.117, 198.481 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 49.557 - 3.788 |
| R-factor | 0.2134 |
| Rwork | 0.211 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1589)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.910 |
| High resolution limit [Å] | 3.700 | 3.770 |
| Rmerge | 0.161 | 0.542 |
| Number of reflections | 129961 | |
| <I/σ(I)> | 4.6 | 1.4 |
| Completeness [%] | 96.3 | 82.6 |
| Redundancy | 2.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% PEG 550 MME, 0.1M HEPES, 1mM ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
