3WVH
Time-Resolved Crystal Structure of HindIII with 25sec soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.782, 143.285, 94.324 |
| Unit cell angles | 90.00, 113.49, 90.00 |
Refinement procedure
| Resolution | 32.910 - 2.540 |
| R-factor | 0.1794 |
| Rwork | 0.176 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4k |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.136 | 0.591 |
| Number of reflections | 63629 | |
| <I/σ(I)> | 12.4 | 2.9 |
| Completeness [%] | 98.4 | 93.78 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10-15% PEG 3350, 300mM NaSCN, 10% v/v Glycerol, 100mM TRIS-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
