3WVF
Crystal structure of YidC from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.729, 76.011, 91.318 |
| Unit cell angles | 78.08, 82.45, 77.55 |
Refinement procedure
| Resolution | 33.881 - 3.200 |
| R-factor | 0.2269 |
| Rwork | 0.221 |
| R-free | 0.27680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bs6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((Phaser-MR)) |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 16696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPID CUBIC PHASE (LCP) | 5.9 | 293 | 25% poly(ethyleneglycol) 600, 100mM MES-NaOH, 50mM potassium thiocyanate, 12mM triglycine, pH 5.9, LIPID CUBIC PHASE (LCP), temperature 293K |
