3WV9
Guanylylpyridinol (GP)- and ATP-bound HcgE from Methanothermobacter marburgensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.008 |
Spacegroup name | P 3 |
Unit cell lengths | 85.000, 85.000, 120.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.690 - 2.750 |
R-factor | 0.16759 |
Rwork | 0.165 |
R-free | 0.21389 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zud |
RMSD bond length | 0.021 |
RMSD bond angle | 2.499 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 | |
High resolution limit [Å] | 2.750 | 12.000 | 2.750 |
Rmerge | 0.105 | 0.031 | 0.870 |
Number of reflections | 25386 | ||
<I/σ(I)> | 15 | 53.27 | 2.47 |
Completeness [%] | 100.0 | 98.1 | 100 |
Redundancy | 7.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 35%(w/v)pentaerythritol ethoxylate 270, 200mM ammonium sulfate, 100mM sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature Room temperatureK |