3WUQ
Structure of the entire stalk region of the dynein motor domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.939, 102.939, 69.069 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.574 - 3.500 |
R-factor | 0.2801 |
Rwork | 0.279 |
R-free | 0.30490 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.121 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.078 | 0.598 |
Number of reflections | 5560 | |
<I/σ(I)> | 29.2 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG3350, 0.2M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |