3WUQ

Structure of the entire stalk region of the dynein motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	102.939, 102.939, 69.069
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.574 - 3.500
R-factor	0.2801
Rwork	0.279
R-free	0.30490
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.121
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.078	0.598
Number of reflections	5560
<I/σ(I)>	29.2	3.3
Completeness [%]	99.9	100
Redundancy		6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% PEG3350, 0.2M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K