3WU4
Oxidized-form structure of E.coli Lon Proteolytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-19 |
Detector | RIGAKU SATURN A200 |
Spacegroup name | P 31 |
Unit cell lengths | 86.391, 86.391, 124.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.410 - 1.700 |
R-factor | 0.156 |
Rwork | 0.152 |
R-free | 0.17700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rr9 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.981 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 111242 | |
<I/σ(I)> | 24.8 | 2.1 |
Completeness [%] | 97.5 | 98.5 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.1M AMMONIUM SULFATE, 0.1M PIPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |