3WT4
Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99999999 |
| Spacegroup name | H 3 |
| Unit cell lengths | 134.187, 134.187, 328.756 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.400 - 2.300 |
| R-factor | 0.14854 |
| Rwork | 0.146 |
| R-free | 0.19632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.103 |
| Number of reflections | 93254 | |
| <I/σ(I)> | 11.08 | |
| Completeness [%] | 94.9 | 99 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | 30% PEG400, 0.1M Tris pH 8.0, 0.2M MgCl2, 0.1mM ZnCl2, VAPOR DIFFUSION, temperature 295K |
