3WT0
Crystal Structure Analysis of Cell Division Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.964330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.032, 70.628, 120.676 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 44.537 - 2.000 |
| R-factor | 0.2093 |
| Rwork | 0.208 |
| R-free | 0.23650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e4f |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.644 |
| Number of reflections | 115953 | |
| <I/σ(I)> | 21.5 | 2.89 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 7.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 20% PEG 4000, 0.1M MES, 0.6M NaCl, pH 6.5, VAPOR DIFFUSION, temperature 293K |
