3WSZ
SorLA Vps10p domain in complex with Abeta-derived peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 163.735, 163.735, 121.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.009 - 3.201 |
R-factor | 0.274 |
Rwork | 0.273 |
R-free | 0.29630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wsy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.049 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 16271 | |
<I/σ(I)> | 29.1 | 4.77 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.6 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 0.1M Tris, 1.41M Sodium Acetate, pH 7.8, VAPOR DIFFUSION, HANGING DROP |