3WSY
SorLA Vps10p domain in complex with its own propeptide fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 41 |
| Unit cell lengths | 158.186, 158.186, 145.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.020 - 3.110 |
| R-factor | 0.19714 |
| Rwork | 0.195 |
| R-free | 0.23174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wsx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.020 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 31428 | |
| <I/σ(I)> | 16.1 | 3.31 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Cacodylate, 1.2M Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
