3WSC
Crystal structure of alginate-binding protein Algp7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Detector technology | CCD |
Collection date | 2012-10-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.157, 97.704, 103.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.497 - 1.992 |
R-factor | 0.2245 |
Rwork | 0.223 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.711 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.990 |
Number of reflections | 35629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.37 | 293 | 18% PEG5000MME, 0.1M Tris-HCl, 0.2M lithium sulfate, pH 7.37, VAPOR DIFFUSION, HANGING DROP, temperature 293K |